Glucoamylase GA-1500 is a powder preparation made from selected strains of Aspergillus niger using submerged fermentation, extraction and refining techniques. GA-1500 has a high conversion rate of starchy substrates into fermentable sugars. It can hydrolyze α-D-1, 4 glucosidic bonds from the non-reducing end of starch one after another. It can also hydrolyze the α-D-1, 6 glucoside branch bonds of starch and cleaves the α-1, 3 bonds, releasing glucose.
GA-1500 has been especially designed for saccharification in ethanol production. Use of GA-1500 provides higher ethanol yields, faster fermentations, and lower residual starch levels.
EC 220.127.116.11, 1,4-alpha-D-glucan glucohydrolase
Light to dark brown powder
150,000 U/g (minimum)
EFFECT OF pH
Effective pH range for GA-1500 is 3.0 to 5.5. For maximum activity, the optimal pH is from 3.5 to 4.5. See Figure 1.
EFFECT OF TEMPERATURE
Generally, the temperature range for GA-1500 is 30 to 65°C. The optimal temperature range is 55 to 60°C. See Figure 2.
PRODUCT APPLICATIONS AND BENEFITS
GA-1500 is used to saccharify liquified mash from various sources including corn, milo, barley, wheat, rice, potatoes and tapioca. The resultant glucose product is fermented by yeast or another appropriate microorganism to yield ethanol.
GA-1500 is designed to ensure the following benefits:
Increased fermentation rate
Increased ethanol yields
Efficient starch/dextrin degradation
Less residual starch in distiller’s grains
Improved performance in pre-saccharification processes
For ground-grain processing plants using simultaneous saccharification fermentation (SSF), GA-1500 may be added directly to the fermenter. For ground-grain dry mill plants and for wet mill plants using a separate pre-saccharification step prior to fermentation, GA-1500 may be added to the cooling mash or liquefied starch when the temperature reaches 55 to 60°C. Temperatures above 65°C can result in loss of activity.
GA-1500 should be dissolved in 25～30°C warm water at a recommended ratio of 1:10 (enzyme: water) prior to use it.
GA-1500 is generally added at a level of 0.7 to 0.9kg/TDS. The actual enzyme requirement is dependent on the raw materials, temperature, reaction time, and pH of the individual plant saccharification/fermentation process, and its desired result.
GA-1500 is packaged in 25kg cardboard drums or kraft paper bags. Customized packaging is available upon request.
This enzyme has been formulated for optimal stability. Typical shelf-life is six months provided it is stored below 25°C in its original packaging, sealed and unopened, protected from the sunlight. Its shelf-life will be eighteen months if it is stored properly at 0～10°C. Prolonged storage and/or adverse conditions such as higher temperatures may lead to a higher dosage requirement.
SAFETY & ENZYME HANDLING
Inhalation of enzyme dust and mists should be avoided. In case of contact with the skin or eyes, promptly rinse with water for at least 15 minutes. For detailed handling information, please refer to the appropriate Material Safety Data Sheet.