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A liquid fungal alpha-amylase fits to both liquefaction and saccharification of starchy materials in starch sugar industry.
Fungal Alpha-Amylase FAA-280 is made from Non-GMO strains of Aspergillus oryzae by submerged fermentation and refining extraction process. It can be used for starch liquefaction and also saccharification to produce maltose and glucose. As an endoamylase, FAA-280 can rapidly hydrolyze the interior α-1, 4 glucosidic linkages of gelatinized starch, amylose and amylopectin solution and produce soluble dextrin and a small quantity of glucose and maltose. It can also produce syrups of high maltose and a small quantity of glucose if the reaction lasts for longer time.
Declared Enzyme: Fungal Alpha-Amylase
Systematic Name: EC 188.8.131.52, 1,4-α-D-Glucan glucanohydrolase
Enzyme Activity: 28,000U/ml (minimum)
Appearance: Sepia brown liquid
Product pH: 5.5 to 7.0
Specific gravity: 1.15 to 1.25 g/ml
EFFECT OF PH
The optimum pH range of this product is 4.8 to 5.4 and starch can be effectively hydrolyzed at pH range 4.0 to 6.6. If the stability of enzyme is emphasized, the reaction should be conducted at pH above 5.5. If the reaction rate is emphasized, the pH should be below 5.5. See Figure 1.
EFFECT OF TEMPERATURE
Optimum temperature range is 45 to 55°C and effective temperature range is 45 to 65°C. Optimum temperature of this product is 50°C. It can efficiently hydrolyze starch at a temperature range of 45 to 55°C. If the content of starch is higher, the reaction temperature can be raised to 65°C. The optimum temperature range is affected by pH, within 45 to 65°C. See Figure 2.
Figure 1: Effect of PH on Activity
|Figure 2: Effect of Temperature on Activity|
Inactivation of FAA-280 can be achieved by raising the temperature to the range of 90 to 100°C and maintaining for approximately 5 to 10 minutes. If the content of starch is higher, this product can be inactivated by keeping the temperature at 80°C for about 30 minutes, or 70°C for about 60 minutes. If inactivation by high temperature is not desirable, another option of inactivation is to decrease the pH below 4.0. Inactivation can also be realized by adjusting pH and temperature at the same time.
This product may be inhibited by several heavy metal ions. Copper and lead ions are strong inhibitors. Inhibitive effects may also be achieved if the content of zinc, nickel and iron ions are high.
This product is applied to saccharify starches from corn, potato, tapioca, wheat, soybean and other resources. It can hydrolyze effectively if the content of starch on the dry substrate basis (DSB) is no more than 50%.
In general, the dosage rate depends on the desirable maltose content of the final syrup and the saccharification time. The typical dosage rate of FAA-280 is in the range of 0.1 to 0.5kg/TDS.
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