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A low pH tolerant Alpha-Amylase with robust activity and super thermal stability for the production of starch sugar.
Alpha-Amylase HT-800 is produced from selected strains of Bacillus licheniformis using submerged fermentation and refining extraction technology. As an endoamylase, HT-800 can quickly decrease the viscosity of gelatinous starch solutions by randomly hydrolyzing starch, glycogen and its degradation products within the alpha-D-1,4 glucosidic bonds to produce soluble dextrins and oligosaccharides.
HT-800 is a robust Heat-stable Alpha-Amylase designed for the production of starch sugar.
Declared Enzyme: Alpha-Amylase
Systematic Name: 1,4-α-D-glucan glucanohydrolase
Activity: 80,000 U/ml (Minimum)
Appearance: Sepia brown Liquid
Product pH: 5.5 to 7.0
Specific gravity: 1.10 to 1.25 g/ml
EFFECT OF PH
HT-800 is active with pH range 5.0 to 7.0, depending on the application. For maximum activity, the optimal pH for HT-800 is 5.4 to 6.0. The exact pH optimum depends on actual processing conditions, including temperature, substrate concentration and time.
EFFECT OF TEMPERATURE
For interval liquefaction, the optimum temperature of HT-800 is above 90°C. It liquefies starch substrates promptly at 95 to 97°C and maintains highly active at 100°C. For consecutive liquefaction, HT-800 demonstrates exceptional thermal stability at a temperature range from 105 to 110°C, maintains its stability for 5 to 7 minutes and keeps on liquefying efficiently.
CONCENTRATION OF CALCIUM
The presence of calcium increases the thermal stability of HT-800. This enzyme requires a very low calcium concentration of 20 to 50 ppm (dry solids basis) to ensure its enzyme stability.
Copper, titanium, and cobalt ions are moderate inhibitors for HT-800. Aluminum, lead and zinc ions act as strong inhibitors.
For industrial application of one-time spray liquefaction, first prepare 30 to 35% w/w starch slurry, adjust pH 5.4 to 6.0, then add this product at a dosage rate of 0.10 to 0.35 kg/TDS. Liquefy at 105 to 107°C for 5 to 7 minutes, flash cooling down to around 97°C, keep on liquefying at temperature above 95°C for 90 to 120 minutes so as to reach required DE value. In case the starch content is above 35%, recommended dosage rate is from 0.35 to 0.4kg/TDS.
Exact dosage rate depends on the actual situation such as starch substrate, liquefaction equipment, processing conditions, etc. To optimize the dosage, it is recommended to conduct a number of liquefaction trials prior to routine use of this product.
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