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Heat-stable Alpha-Amylase

Heat-stable Alpha-Amylase HT-800

A low pH tolerant Alpha-Amylase with robust activity and super thermal stability for the production of starch sugar.

Product Specifications



Alpha-Amylase HT-800 is produced from selected strains of Bacillus licheniformis using submerged fermentation and refining extraction technology. As an endoamylase, HT-800 can quickly decrease the viscosity of gelatinous starch solutions by randomly hydrolyzing starch, glycogen and its degradation products within the alpha-D-1,4 glucosidic bonds to produce soluble dextrins and oligosaccharides.

HT-800 is a robust Heat-stable Alpha-Amylase designed for the production of starch sugar.


           Declared Enzyme:            Alpha-Amylase

           Systematic Name:           1,4-α-D-glucan glucanohydrolase

           Activity:                            80,000 U/ml (Minimum)

           Appearance:                    Sepia brown Liquid

           Product pH:                     5.5 to 7.0

           Specific gravity:              1.10 to 1.25 g/ml


HT-800 is active with pH range 5.0 to 7.0, depending on the application. For maximum activity, the optimal pH for HT-800 is 5.4 to 6.0. The exact pH optimum depends on actual processing conditions, including temperature, substrate concentration and time.


For interval liquefaction, the optimum temperature of HT-800 is above 90°C. It liquefies starch substrates promptly at 95 to 97°C and maintains highly active at 100°C. For consecutive liquefaction, HT-800 demonstrates exceptional thermal stability at a temperature range from 105 to 110°C, maintains its stability for 5 to 7 minutes and keeps on liquefying efficiently.


The presence of calcium increases the thermal stability of HT-800. This enzyme requires a very low calcium concentration of 20 to 50 ppm (dry solids basis) to ensure its enzyme stability.


Copper, titanium, and cobalt ions are moderate inhibitors for HT-800. Aluminum, lead and zinc ions act as strong inhibitors.


For industrial application of one-time spray liquefaction, first prepare 30 to 35% w/w starch slurry, adjust pH 5.4 to 6.0, then add this product at a dosage rate of 0.10 to 0.35 kg/TDS. Liquefy at 105 to 107°C for 5 to 7 minutes, flash cooling down to around 97°C, keep on liquefying at temperature above 95°C for 90 to 120 minutes so as to reach required DE value. In case the starch content is above 35%, recommended dosage rate is from 0.35 to 0.4kg/TDS.

Exact dosage rate depends on the actual situation such as starch substrate, liquefaction equipment, processing conditions, etc. To optimize the dosage, it is recommended to conduct a number of liquefaction trials prior to routine use of this product.

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