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Heat-stable Alpha-Amylase

Heat-stable Alpha-Amylase HA-580FG

A food grade heat-stable alpha-amylase produced from selected strains of Bacillus licheniformis  especially designed for the production of alcohol.

Product Specifications



DESCRIPTION 

HA-580FG is a food grade heat-stable alpha-amylase produced from selected strains of Bacillus licheniformis through submerged fermentation and refining extraction processes. It is an endoamylase which can rapidly decrease the viscosity of gelatinous starch solutions by randomly hydrolyzing starch, glycogen and its degradation products within the alpha-D-1,4 glucosidic bonds to produce soluble dextrins and oligosaccharides.

 

HA-580FG is a high concentrated formulation specially designed for the production of alcohol.

 

PRODUCT CHARACTERISTICS  

Declared Enzyme:

Alpha-Amylase

Systematic Name:

1,4-α-D-glucan glucanohydrolase

Activity:

400,000 U/ml (minimum)

Appearance:

Sepia brown liquid

Product pH:

5.0 to 7.0

Specific gravity: 

1.10 to 1.25 g/ml

 

EFFECT OF PH 

HA-580FG is active over a wide pH range. It can work at pH ranging from 5.2 to 6.5 with its optimal pH range from 5.4 to 6.0. The exact pH optimum depends on the actual processing conditions, including temperature, substrate concentration and processing time.

 

EFFECT OF TEMPERATURE

For interval liquefaction, HA-580FG works at temperatures from 80 to 100°C with it optimum temperature range from 83 to 86°C. HA-580FG can liquefy starch substrates promptly at 90 to 95ºC and maintain highly active at 100ºC.


For consecutive liquefaction, HA-580FG demonstrates its exceptional thermo-stability at a temperature range of 105 to 110ºC and keeps on liquefying efficiently for 5 to 7 minutes.

 

INHIBITORS

Copper, titanium, and cobalt ions are moderate inhibitors for HA-580FG whilst aluminum, lead and zinc ions are strong inhibitors.

 

USAGE GUIDELINES

First adjust pH to 5.7 to 5.9, and then add HA-580FG. After this enzyme is added, heat the slurry to liquefy at a temperature range from 83 to 86ºC for 90 to 150 minutes. Final DE value should be maintained at a range from 10 to 12.


Dosage of HA-580FG depends on the actual processing conditions such as kind of starch, substrate concentration, processing time, temperature, pH, etc. To optimize the dosage, it is recommended to conduct a number of liquefaction trials prior to routine use of this product. A good starting point for dosage rate is 0.05 to 0.1kg/TDS.


PACKAGING, STORAGE & SHELF-LIFE

HA-580FG is packaged in 25kg or 30kg food-grade HDPE plastic drums, or 1125kg IBC totes. Customized packaging is available upon request.

 

Typical shelf-life is six months if it is stored below 25°C in its original packaging, sealed and unopened, protected from direct sunlight. Its shelf-life will be eighteen months if this product is stored properly at 0~10°C. HA-580FG has been formulated for optimal stability. Prolonged storage and/or adverse conditions such as higher temperatures may lead to a higher dosage requirement, thus should be avoided.

 

SAFE HANDLING OF ENZYMES 

Inhalation of enzyme dust and mists should be avoided. In case of contact with skin or eyes, promptly rinse with water for at least 15 minutes. For detailed handling information, please refer to the Material Safety Data Sheet.


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