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Enzymes for Ethanol

Heat-stable Alpha-Amylase HS-400

Made from Non-GMO strains of Bacillus licheniformis, enables better liquefaction and fermentation results.

Product Specifications


Heat-stable Alpha-Amylase HS-400 is produced from Non-GMO strains of Bacillus licheniformis through submerged fermentation and refining extraction process. It is an endoamylase that can rapidly decrease the viscosity of a gelatinous starch solution by randomly hydrolyzing starch, glycogen and its degradation products within the α-D-1, 4 glucosidic bonds to produce soluble dextrins and oligosaccharides.

HS-400 is applied  to the starch liquefaction in ethanol industry.


Declared Enzyme:               Alpha-Amylase

Systematic Name:               1,4-α-D-glucan glucanohydrolase

Activity:                                  40,000 U/ml (minimum)

Appearance:                        Sepia brown liquid

Product pH:                          5.0 to 7.0

Specific gravity:                  1.10 to 1.25 g/ml


HS-400 is active over a wide range of pH, depending on the application. For maximum activity, its optimal pH is from 6.0 to 6.4. At a temperature of 25°C, its stable pH range is 5.0 to 10.0. The pH optimum depends on actual process conditions including temperature, substrate concentration and processing time.


Optimum temperature of HS-400 for interval liquefaction is above 90°C. It liquefies starch substrates efficiently at 95 to 97°C and remains highly active at 100°C. HS-400 demonstrates exceptional thermal stability at temperaturesfrom 105 to 110°C, maintains its stability for 5 to 7 minutes and keeps on liquefying efficiently. At a temperature above 105°C, the enzyme stability decreases rapidly. The exact temperature optimum depends on actual processing conditions including pH, substrate concentration and processing time.


The presence of calcium increases the thermal stability of HS-400. This enzyme requires a very low calcium concentration and calcium additions of 50 to  70 ppm (dry solids basis) are sufficient to ensure enzyme stability.


Copper, titanium, and cobalt ions are moderate inhibitors for HS-400. Aluminum, lead and zinc ions act as strong inhibitors.


In general, first adjust the pH at 6.0 to 6.4 and then add calcium (50 to 70 ppm dry solids basis) prior to  adding HS-400. Calcium is not needed when extremely hard water (above 10 German degrees) is used. After the enzyme is added, heat the slurry to liquefy.


Dosage of HS-400 depends on the actual conditions such as starch substrate, application, substrate concentration and other processing conditions. To optimize the dosage, it is recommended to conduct a number of liquefaction trials prior to the routine use of this product. A good starting point for dosage rate is 0.10 to 0.25kg /TDS.

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