A cost-effective alpha amylase derived from Non-GMO strains of Aspergillus oryzae, enables better liquefaction and saccharification of starches in ethanol production.
Fungal Alpha-Amylase FAA-400 is made from Non-GMO strains of Aspergillus oryzae through submerged fermentation and refining extraction proecess. FAA-400 is an endoamylase which can rapidly hydrolyze the interior α-1, 4 glucosidic linkages of gelatinized starch, amylose and amylopectin solution and produce soluble dextrin and a small quantity of glucose and maltose.
FAA-400 can be used to liquefy and saccharify starches in ethanol industry.
Declared Enzyme: Fungal Alpha-Amylase
Systematic Name: EC 22.214.171.124.1,4-α-D-Glucan glucanohydrolase
Activity: 28,000 U/ml (minimum)
Appearance: Sepia brown liquid
Product pH: 5.5 to 7.0
Specific gravity: 1.15 to 1.25 g/ml
Use of FAA-400 provides the following benefits to ethanol producers:
■ Lower total residual sugars at drop
■ Increase ethanol production
■ Lower residual DP4+ material due to increased conversion
■ Lower residual starch in DDGS
■ Lower glycerol production due to a more proportional glucose/maltose profilereducing osmotic stress
EFFECT OF PH
The optimum pH range of FAA-400 is 4.8 to 5.4 and starch can be effectively hydrolyzed at pH range 4.0 to 6.6. Typical effect of pH on enzyme activity is shown in Figure 1.
EFFECT OF TEMPERATURE
Optimum temperature ranges from 45 to 55°C and effective temperature range is from 30 to 65°C. Typical effect of Temperature is shown in Figure 2.
|Figure 1: Effect of pH on Activity||
Figure 2: Effect of temperature on Activity
Inactivation can be achieved by raising the temperature to 90 to 100°C and maintaining for approximately 5 to 10 minutes. If the content of starch is higher, this product can be inactivated by keeping the temperature at 80°C for about 30 minutes, or 70°C for about 60 minutes. If inactivation by high temperature is not desirable, another option of inactivation is to decrease the pH below 4.0. Inactivation can also be realized by adjusting pH and temperature at the same time.
FAA-400 can be inhibited by several heavy metal ions. Copper and lead ions are strong inhibitors. Inhibitive effects may also be achieved if the content of zinc, nickel and iron ions are high.
In general, the dosage rate depends on the actual processing conditions. The typical dosage rate of FAA-400 is in the range of 0.04 to 0.08kg/TDS.
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