A food-grade Fungal Alpha-Amylase that can give better handling properties, fine crumb structure, desirable elasticity, softness and extended shelf-life.
Fungal Alpha-Amylase FAA-5000 is a high activity fungal alpha-amylase derived from non-genetic modified strains of Aspergillus oryzae using submerged fermentation and refining extraction techniques.
FAA- 5000 has been specifically designed for the baking industry as a bread improver and alpha-amylase supplement for flour. As an endoamylase, FAA-5000 can rapidly hydrolyze the interior α-1, 4 glucosidic linkages of gelatinized starch, amylose and amylopectin solutions to produce soluble dextrins and small quantities of maltose and glucose. Long-time reaction will result in a small amount of syrup which contains high maltose and glucose.
Declared Enzyme: Fungal Alpha-Amylase
Systematic Name: EC 126.96.36.199, 1,4-alpha-D-Glucan glucanohydrolase
Activity: 50,000 U/g (minimum)
Appearance: Light ivory Powder
Moisture: 8% (Maximum)
EFFECT OF PH
FAA-5000 can effectively hydrolyze starches at a pH range from 4.0 to 6.5, with the optimum pH range being 4.8 to 5.4. If greater enzyme stability is preferred, the reaction should be conducted at a pH above 5.5; if a faster reaction rate is desired, the pH should be kept below 5.5. Its pH optimum will depend on several process variables such as time, temperature, substrate concentration, and dough moisture levels. Figure 1 shows the effect of pH on FAA-5000 activity.
EFFECT OF TEMPERATURE
FAA-5000 can effectively hydrolyze starch at temperatures from 45°C to 55°C, with its optimum temperature at 50°C. Its optimum temperature depends on the actual processing conditions such as time, pH, substrate concentration, moisture, etc. Figure 2 shows the effect of temperature on FAA-5000 activity.
The temperature versus activity profile of FAA-5000 matches well with the demand for sugars needed by the yeast during the dough fermentation. As the dough begins to ferment, the activity of the FAA-5000 and the yeast will be enhanced as the temperature rises (i.e. the Fungal Alpha-Amylase constantly hydrolyzes the soluble starchescontained in the dough and produces monosaccharides to support yeast proliferation, thus increasing the fermentation speed and improving the dough quality). When fermentation of the dough is completed and the semi-products begin to be steamed or baked, the yeast will become inactivated at 60°C and FAA-5000 will lose its activity as well (thus avoiding excessive saccharification of the dough which might otherwise lead to undesirable stickiness of finished products).
Inactivation of FAA-5000 can be achieved by maintaining the temperature at 90 to100°C for 5 to 10 minutes. If the starch content is high, FAA-5000 can be inactivated by maintaining the temperature at 80°C for about 30 minutes, or at 70°C for about 60 minutes.
FAA-5000 can be used to increase loaf volume, improve texture and appearance of bread, and extend shelf life of baked products.
DOSAGE AND ADDITION METHOD
The recommended dosage is 0.2 to 0.5kg per ton of flour. The exact dosage varies among bakeries depending on the type of flour, the specific formulation, the type of equipment and process. It is recommended to conduct baking trials to determine the optimal dosage.
It is important that proper dispersion and mixing of the FAA-5000 is achieved to obtain optimal efficiency of the enzyme performance in the process. Due to the small dosage required, it is recommended to premix the FAA-5000 in a dry state first with a small amount of flour, and then add it to the batch over a long enough period of time so as to ensure even mixing with the flour. It is also acceptable to add the enzyme directly to the dough.
Due to the small dosage required, in order to ensure a proper dispersion and mixing, it is recommended to dilute FAA-5000 with a small amount of starch first, and then add it to the batch to mix with the flour evenly.
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