A protease derived from robust strains of Aspergillus niger, capable to catalyze hydrolysis under acidic conditions, promote yeast growth, maximize fermentation and enhance alcohol yields in a cost-effective way.
Acid Protease AP-530 is produced from robust strains of Aspergillus niger through submerged fermentation and refining extraction process. It can catalyze hydrolysis of proteins under acidic conditions. AP-530 is applicable to hydrolysis of proteins with acidic medium.
Declared Enzyme: Acid Protease
Systematic Name: EC 184.108.40.206
Activity: 150,000 U/ml (minimum)
Appearance: Black brown Liquid
Product pH: 3.5 to 6.0
Specific gravity: 1.15 to 1.25 g/ml
EFFECT OF PH
AP-530 works with pH ranges from 2.0 to 6.0 with its optimal pH from 2.5 to 3.5.
EFFECT OF TEMPERATURE
AP-530 can work effectively from 30 to 65°C with an optimal temperature range from 40 to 50°C.
EFFECT OF METAL IONS
AP-530 is activated by Mn2+, Ca2+ and Mg2+, and inhibited by Cu2+, Hg2+ and Al3+.
Use of AP-530 in fermentation process offers the following benefits:
1) Promote raw material particles dissolved
AP-530 can hydrolyze the proteins of the raw materials, thus destroy the interstitial cell wall structure of starch granules, so as to release the starch wrapped in the granules, which is conducive to saccharification.
2) Promote the growth of yeast with reproduction
Free amino nitrogen (FAN) is the most effective nitrogen source for yeast. Adding AP-530 during alcoholic fermentation can effectively hydrolyze proteins and peptides in the raw materials so as to generate FAN that yeasts can absorb, thus promote the growth of yeasts, increase the quantities of yeasts in the fermented mash, and shorten the fermentation cycle.
3) Beneficial to high concentration fermentation
In high concentration fermentation of alcohol, it is mainly nutritional deficiencies rather than the accumulation of alcohol that leads to the decrease of fermentation activity. Free amino acids (FAN) can not only shorten the fermentation cycle but also increase the output of alcohol, improve ethanol tolerance and survival rate of yeasts. Therefore, AP-530 is the best nutrient for yeast growth and accelerator for fermentation.
In intermittent fermentation, it should be added at one time when the fermentation tank is filled with one third materials. In continuous fermentation, it should be added consecutively depending on the amount of saccharification mash fed at the inlet of fermentation tank.
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