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Enzymes for Alcohol & Brewing

Neutral Protease NP-100

A liquid neutral protease for protein hydrolysis in food industry.

Product Specifications



Neutral Protease NP-100 is produced from non-genetic modified strains of Bacillus subtilis through submerged fermentation, extraction and refining process. NP-100 catalyzes protein hydrolysis in neutral environment and generates peptide and a small quantity of amino acids.


Declared Enzyme:


EC Number:



Black brown Liquid


100,000 U/ml (minimum)

Product pH:
4.0 to 7.0



NP-100 works at pH ranging from 6.8 to 8.0 with its optimum pH range from 7.0 to 7.5. Inactivation of NP-100 can be achieved quickly at pH below 5.0 or above 9.0.


NP-100 works at temperatures from 45 to 50°C. Its optimum temperature is 50°C. It is inactivated quickly at temperatures above 60°C.


1) Hydrolysis of animal protein

NP-100 can be used to hydrolyze meat, fish, terrapin, pig pancreas, silkworm pupae, etc. to produce peptide and amino acids.

2) Hydrolysis of plant protein 

Adding NP-100 into soy milk or instant soy milk powder can considerably increase the yield of proteins and the solid content.

3) Beer Production 
In beer production, inadequate quality of malt or large content of auxiliaries will lead to insufficient α-amino acid, thus result in insufficient yeast propagation, abnormal fermentation and unwanted diacetyl content in beer. NP-100 can help to increase α-amino acid content in beer. Dosage rate depends on the original wort content.

4) Soy Sauce   
In soy sauce production, proteolysis is the key to maximize raw material utilization and improve product quality. Use of this enzyme can partly replace Koji will not only increase the utilization rate of the raw materials but also maintain the desirable flavor and quality of final products. 

5) Treatment of animal blood 
Use of NP-100 in the treatment of red cell of blood can produce decolorized and soluble blood hydrolyte, which can be used in the production of calcium supplements, oral liquid nutritional supplements, food additives for food and beverage so as to enhance food nutrition. 

NP-100 is packaged in 25kg, 30kg HDPE drums or 1125 kg IBC totes. Customized packaging is also available upon request. 

Typical shelf-life is six months provided it is stored 0∼ 10°C in its original packaging, sealed and unopened, protected from sunlight. NP-100 has been formulated for optimal stability. Prolonged storage and/or adverse conditions such as higher temperatures may lead to a higher dosage requirement, therefore should be avoided. 

Inhalation of enzyme dust and mists should be avoided. In case of contact with skin or eyes, promptly rinse with water for at least 15 minutes. For detailed handling information, please refer to the Material Safety Data Sheet. 

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