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Glucoamylase ETHMEI FD

ETHMEI FD is a high concentration glucoamylase that especially designed for saccharification in production of fuel ethanol.

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Product Specifications


ETHMEI FD is a high concentration glucoamylase produced from strains of Aspergillus niger through submerged fermentation, extraction and refining processes. It has a high conversion rate in turning starchy substrates into fermentable sugars. In addition to hydrolyze α-D-1, 4 glucosidic bonds from the non-reducing end of starch one after another, ETHMEI FD can also hydrolyze α-D-1, 6 glucoside branch bonds of starch and cleaves α-1, 3 bonds to release glucose.

ETHMEI FD has been especially designed for saccharification in production of fuel ethanol.

Product Characteristics

Declared Enzyme:


Systematic Name:

EC3.2.1.3, 1,4-alpha-D-glucan glucohydrolase


Light to dark brown liquid


400,000 U/ml

Product pH

3.0 to 5.0

Specific gravity:

1.10 to 1.20 g/ml

Effect of pH

Effective pH range for ETHMEI FD is 3.0 to 5.5. For maximum activity, the optimal pH is from 3.5 to 4.5. See Figure 1.

Effect of Temperature

Generally, the temperature range for ETHMEI FD is 30 to 65℃. The optimal temperature range is 55 to 60°C. See Figure 2.

Application & Benefits

ETHMEI FD is used to saccharify liquified mash from various sources including corn, milo, barley, wheat, rice, potatoes and tapioca. The resultant glucose product is fermented by yeast or another appropriate microorganism to yield ethanol.

ETHMEI FD is designed to ensure the following benefits:

1)  Increased fermentation rate

2)  Increased ethanol yields

3)  Efficient starch/dextrin degradation

4)  Less residual starch in distiller’s grains

5)  Improved performance in pre-saccharification processes

For ground-grain processing plants using simultaneous saccharification fermentation (SSF), ETHMEI FD may be added directly to the fermenter. For ground-grain dry mill plants and for wet mill plants using a separate pre-saccharification step prior to fermentation, ETHMEI FD may be added to the cooling mash or liquefied starch when the temperature reaches 55 to 60°C. Temperatures above 65°C can result in loss of activity.

Dosage Guidelines

ETHMEI FD is generally added at a level of 0.26 to 0.34kg/TDS. The actual enzyme requirement is dependent on the raw materials, temperature, reaction time, and pH of the individual plant saccharification/fermentation process, and its desired result.

Packaging, Storage & Shelf-life

Standard packaging is 25kg, 30kg HDPE drums or 1125 kg IBC totes.

Typical shelf-life is six months if this product is stored below 25°C in its original packaging, sealed and unopened, protected from sunlight. Its shelf-life will be eighteen months if this product is stored properly at 0 ∼10°C. This product has been formulated for optimal stability. Prolonged storage and/or adverse conditions such as higher temperature may lead to a higher dosage requirement, therefore should be avoided.

Safe Handling of Enzymes

Inhalation of enzyme dust and mists should be avoided. In case of contact with the skin or eyes, promptly rinse with water for at least 15 minutes. For detailed handling information, please refer to the Safety Data Sheet.

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