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Glucoamylase

Glucoamylase HGA-101PC

Glucoamylasecan hydrolyze alpha-1,4glucosidic linkages from the non-reduction end of starches to release glucose, whilst Pullulanase can specifically cleave the alpha-1, 6 glycosidic bond of the branching point of amylopectin, and cut out the entire branch structure to form amylose. Thus HGA-101PC has a higher conversion rate from starch to glucose.


This compound enzyme product has been specially designed for the production of starch sugar.

Product Specifications


DESCRIPTION

HGA-101PCis a Glucoamylase / Pullulanse blended enzyme. It contains robust Glucoamylase and starch debranching enzyme-Pullulanase. The Glucoamylase is produced from controlled fermentation of selected strains of Aspergillus niger ,whilst the Pullulanase is produced from robust strains of Bacillus licheniformis. Glucoamylasecan hydrolyze alpha-1,4glucosidic linkages from the non-reduction end of starches to release glucose, whilst Pullulanase can specifically cleave the alpha-1, 6 glycosidic bond of the branching point of amylopectin, and cut out the entire branch structure to form amylose. Thus HGA-101PC has a higher conversion rate from starch to glucose.


This compound enzyme product has been specially designed for the production of starch sugar.


PRODUCT CHARACTERISTICS

Glucoamylase Activity:

120,000U/ml (minimum)

Pullulanase Activity:

800BU/ml (minimum)

Appearance:

Yellow brown liquid

Product pH:

3.0 to 5.0

Specific Gravity:

1.10 to 1.25 g/ml

Effect of pH

HGA-101PC can work at pH range from 3.0 to 5.5. For maximum activity, the optimal pH range is 4.0 to 4.5, with an optimum pH at 4.2(See Figure 1).


Figure 1: Effect of pH on Pullulanase and Glucoamylase Activity at 60°C


Effect of Temperature

HGA-101PC can work at temperatures from 30 to 65°C. For maximum activity, its optimal temperature is from 60 to 62°C. Normally, this product will be completely inactivated if the saccharified liquid is boiled for 5 minutes at 80°C(See Figure 2).




Benefits for Starch Sugar Industry

Use of HGA-101PC ensures the following benefits for starch sugar industry:

ü  Greater conversion rate from starch to glucose

ü  Maximized utilization of raw materials

ü  Suitable for the production of glucose with DX≥96%

ü  Capable to work with higher concentration substrate

ü  Stable activity ensures stable dosage rate, no need to adjust dosage for each batch

Usage Guidelines

Add this product into liquefied starches of substrates such as corn, wheat, barley, tapioca, rice, potatoes, etc. can convert the starches into glucose.

Recommended optimal temperature for industrial operations is 60 to 62°C, pH4.2 to 4.4.

First, adjust pH, and then add this product. Dosage rate depends on the type of raw materials and actual processing conditions. It is recommended to conduct several trials to determine the optimal dosage rate. A generally recommended dosage rate is 0.35 to 0.6kg / TDS. Saccharification process should be around 32∼48 hours.



Packaging, Storage & Shelf-life

²  Standard packaging is 25kg, 30kg food-grade HDPE drums or 1125 kg IBC totes.

²  Customized packaging is available upon request.

²  Typical shelf-life is six months if it is stored below 25°C in its original packaging, sealed and unopened, protected from sunlight.

²  Its shelf-life will be eighteen months if this product is stored properly at 0∼10°C.

²  HGA-101PC has been formulated for optimal stability. Prolonged storage and/or adverse conditions such as higher temperature may lead to a higher dosage requirement, therefore should be avoided.



Safe Handling of Enzymes

Inhalation of enzyme dust and mists should be avoided. In case of contact with skin or eyes, promptly rinse with water for at least 15 minutes. For detailed handling information, please refer to the Safety Data Sheet.






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