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Acid Protease

Acid Protease AP-150

A robust protease suitable for high concentration fermentation in ethanol production, capable to promote yeast growth and enable more ethanol yields in a cost-effective way.

Product Specifications


Acid Protease AP-150 is produced from robust strains of Aspergillus niger through submerged fermentation and extraction and refining technique. It can catalyze the hydrolysis of proteins under acidic conditions. AP-150 is applicable to hydrolysis of proteins with acidic medium.

AP-150 is suitable for the production of ethanol.

Declared Enzyme:           Acid Protease 
EC Number:                    EC
Activity:                           150,000 U/ml (minimum)
Appearance:                   Black brown Liquid
Product pH:                    3.5 to 6.0
Specific gravity:             1.15 to 1.25 g/ml

AP-150 can work at pH range from 2.0 to 6.0 with its optimal pH from 2.5 to 3.5.

AP-150 can work at temperatures from 30 to 65°C with its optimal temperature from 40 to 50°C.

AP-150 is activated by Mn2+, Ca2+ and Mg2+, and inhibited by Cu2+, Hg2+ and Al3+.


Use of AP-150 in fermentation process offers the following benefits to the production of ethanol:

1) Promote raw material particles dissolved
AP-150 can hydrolyze the proteins of the raw materials, thus destroy the interstitial cell wall structure of starch granules, so as to release the starch wrapped in the granules, which is conducive to saccharification.

2) Promote the growth of yeast with reproduction
Free amino nitrogen (FAN) is the most effective nitrogen source for yeast. Adding AP-150 during alcoholic fermentation can effectively hydrolyze proteins and peptides in the raw materials so as to generate FAN that yeasts can absorb, thus promote the growth of yeasts, increase the quantities of yeasts in the fermented mash, and shorten the fermentation cycle.
3) Beneficial to high concentration fermentation
In high concentration fermentation of alcohol, it is mainly nutritional deficiencies rather than the accumulation of alcohol that leads to the decrease of fermentation activity. Free amino acids (FAN) can not only shorten the fermentation cycle but also increase the output of alcohol, improve ethanol tolerance and survival rate of yeasts. Therefore, AP-150 is the best nutrient for yeast growth and accelerator for fermentation.

In intermittent fermentation, it should be added at one time when the fermentation tank is filled with one third materials. In continuous fermentation, it should be added consecutively depending on the amount of saccharification mash fed at the inlet of fermentation tank.


Typical shelf-life is six months if this product is stored at 0∼ 10°C in its original packaging, sealed and unopened, protected from the sunlight. Prolonged storage and/or adverse conditions such as higher temperatures may lead to a higher dosage requirement, thus should be avoided.

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