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A protease derived from robust strains of Aspergillus niger, capable to catalyze hydrolysis under acidic conditions, promote yeast growth, maximize fermentation and enhance alcohol yields in a cost-effective way.
Acid Protease AP-530 is produced from selected strains of Aspergillus niger through submerged fermentation and refining extraction processes. It can catalyze hydrolysis of proteins under acidic conditions. AP-530 is applicable to hydrolysis of proteins with acidic medium.
Declared Enzyme: Acid Protease
Systematic Name: EC 188.8.131.52
Activity: 150,000 U/ml (minimum)
Appearance: Black brown Liquid
Product pH: 2.5 to 5.0
Specific gravity: 1.15 to 1.25 g/ml
EFFECT OF PH
AP-530 works with pH ranges from 2.0 to 6.0 with its optimal pH range from 2.5 to 3.5.
EFFECT OF TEMPERATURE
AP-530 can work effectively from 30 to 65°C with an optimal temperature range from 40 to 50°C.
EFFECT OF METAL IONS
AP-530 is activated by Mn2+, Ca2+ and Mg2+, and inhibited by Cu2+, Hg2+ and Al3+.
Use of AP-530 in fermentation process offers the following benefits:
1) Enable raw material particles dissolved easily
AP-530 can hydrolyze the proteins of the raw materials, thus destroy the interstitial cell wall structure of starch granules, so as to release the starch wrapped in the granules, which is conducive to saccharification.
2) Promote the
growth and reproduction of yeasts
Free amino nitrogen (FAN) is the most effective nitrogen source for yeast. Adding AP-530 during alcoholic fermentation can effectively hydrolyze proteins and peptides contained in raw materials so as to generate FAN that yeasts can absorb, thus promote the growth of yeasts, increase quantities of yeasts in fermented mash, thus shorten fermentation cycle.
3) Beneficial to high concentration fermentation
In high concentration
fermentation of alcohol, it is mainly nutritional deficiencies rather than the
accumulation of alcohol that leads to the decrease of fermentation activity.
Free amino acids (FAN) can not only shorten the fermentation cycle but also
increase the output of alcohol, improve ethanol tolerance and survival rate of
yeasts. Therefore, AP-530 is the most desirable nutrient for yeast growth and
accelerator for fermentation.
In intermittent fermentation process, AP-530 should be added at one time when the fermentation tank is filled with one third of materials. In continuous fermentation process, this enzyme should be added consecutively depending on the quantities of saccharification mash fed at the inlet of fermentation tank.
Recommended dosage of AP-530 is 0.001 to 0.01% w/w (dry solids basis). Actual dosage rate should be depending on the raw materials and process of production.
PACKAGING, STORAGE & SHELF-LIFE
AP-530 is packaged in 25kg, 30kg HDPE drums or 1125kg IBC totes. Customized packaging is available upon request.
Typical shelf-life is six months if this product is stored below 25°C in its original packaging, sealed and unopened, protected from the sunlight. Its shelf-life will be twelve months if it is stored properly at 0∼10°C. AP-530 has been formulated for optimal stability. Prolonged storage and/or adverse conditions such as higher temperatures may lead to a higher dosage requirement, and should be avoided.
SAFE HANDLING OF ENZYMESInhalation of enzyme dust and mists should be avoided. In case of contact with the skin or eyes, promptly rinse with water for at least 15 minutes. For detailed handling information, please refer to the Material Safety Data Sheet.
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